Manual covers DNA isolation, purification, and quantification methods. Provides protocols for gel electrophoresis and Southern blotting. Discusses plasmid vectors and transformation methods. Covers high-capacity vectors like Bacterial Artificial Chromosomes. Explains RNA extraction and analysis techniques
Big data refers to large, unmanageable amounts of data exceeding traditional storage limits. Digital universe grew from 130 exabytes in 2005 to 40,000 by 2020. Healthcare generates massive amounts of data from various sources
NGS determines DNA/RNA sequence for genetic variation analysis. Introduced in 2005 as massively-parallel sequencing. Enables simultaneous analysis of hundreds to thousands of genes. Offers higher accuracy and lower sample requirements than Sanger sequencing
Multiomics combines multiple omics technologies to study life in concerted way. ASCO defines panomics as combining cell and body function data with patient information. More than 99 software and databases available for multiomic data analysis
I-Mutant2.0 predicts protein stability changes upon single-site mutations. Tool trained on ProTherm database, most comprehensive protein mutation database. Predicts stability change from structure (80%) or sequence (77%)
Contains ~280,000 human non-coding RNA genes from RNAcentral transcripts. Includes ~120,000 long non-coding RNAs, including ~100,000 novel genes. LncRNA genes uniformly distributed across nuclear chromosomes. Provides 131 pathways for 38 lncRNA genes