A549 cells were isolated from lung cancer tissue of a 58-year-old male. Cells are positive for keratin and express G6PD, B. Can synthesize high-percentage lecithin using cytidine diphosphocholine pathway
Hep G2 is a hepatocellular carcinoma cell line from a 15-year-old male. Expresses insulin and insulin-like growth factor II. Also known as hepatoblastoma cell line. No evidence of Hepatitis B virus genome
Trypsin is a serine protease found in small intestine, breaking protein chains. Discovered in 1876 by Wilhelm Kühne, named from Greek 'thrýpto' meaning 'I break'. Produced as inactive trypsinogen in pancreas, activated by enterokinase. Catalyzes hydrolysis of peptide bonds, mainly at lysine or arginine carboxyl side
Growth medium supports cell proliferation in microorganisms and small plants. Two main types: cell culture and microbiological culture. Most common media are nutrient broths and agar plates
Petri dish was invented by Robert Koch in 1881 as precursor method. Julius Petri made final modifications in 1887 at Berlin University. Koch's method used agar medium developed by Walther Hesse. Petri's dish eliminated transfer steps and reduced contamination risk
Tissue culture involves growing tissues or cells in artificial media. Term coined by American pathologist Montrose Thomas Burrows. Wilhelm Roux established basic principle in 1885. Haberlandt first pointed out plant tissue culture possibilities in 1902