Kits enable real-time PCR and two-step RT-PCR with SYBR Green or sequence-specific probes. Allow detection of up to 5 targets in single tube. Master mixes contain HotStarTaq DNA Polymerase for stringent hot start. Available in two formats: Multiplex PCR with ROX dye or NoROX
Manual covers DNA isolation, purification, and quantification methods. Provides protocols for gel electrophoresis and Southern blotting. Discusses plasmid vectors and transformation methods. Covers high-capacity vectors like Bacterial Artificial Chromosomes. Explains RNA extraction and analysis techniques
Sequence quality must be checked before primer selection. Repbase database helps identify vector contaminants and repeats. Low-quality bases should be converted to N's or excluded. Primer peaks often occur at sequence ends
Brazilians are citizens of Brazil, regardless of ethnicity. Citizenship requires birth in Brazil or registration with Brazilian parents. Foreigners can apply after 4 years in Brazil and speak Portuguese
Anatolian Turks are a mix of West, Central, and Northeast Asian ancestry. Most Turks have deep roots in Turkey, descended from Armenians and Hittites. Some Turks have recent Balkan and Caucasus ancestry, fully assimilated. Some Turks have recent Jewish ancestry
RNA-seq enables detailed analysis of individual cells' transcriptomes. scRNA-seq reveals cellular heterogeneity and rare cell populations. Technique allows study of unique cells like T lymphocytes and neurons