Western blot identifies proteins using antibodies separated by size in gel electrophoresis. Proteins migrate from gel to membrane through electrical current. Membrane can be made of nitrocellulose or PVDF
Tests look for specific antibodies in blood to detect immune system responses. Antibodies protect against pathogens like viruses and bacteria. Results may indicate past infections, vaccinations, or autoimmune diseases
Western blot is a protein detection technique using antibodies. Proteins are separated on SDS-PAGE gels and transferred to membrane. Western blotting involves transfer, blocking, and detection steps
DAT determines if red blood cells are covered with antibodies. Antibodies attach to RBCs, causing their destruction
ENAs are over 100 soluble cytoplasmic and nuclear antigens. Six main proteins: Ro, La, Sm, RNP, Scl-70, Jo1. Can be removed from cell nuclei using saline. Associated with spliceosomes and nucleosomes complexes
Western blot detects specific proteins in tissue samples using antibodies. Technique combines size separation, protein transfer, and antibody detection. Name derived from Southern blot, independently invented in 1979