Marvel's Avengers Assemble 2. sezonunda yer alan bazı karakterler: Ana karakterler: Iron Man, Captain America, Thor, Hulk, Hawkeye, Falcon, Black Widow. Antagonistler: Infinity Stones arcı: Thanos, Red Skull, Loki, Hela, Winter Soldier. Ultron Outbreak arcı: Ultron, Justin Hammer, Absorbing Man, Titania, M.O.D.O.K., Crimson Dynamo. Squadron Supreme arcı: Nighthawk, Hyperion, Power Princess, Speed Demon, Doctor Spectrum, Nuke. Destekleyen karakterler: J.A.R.V.I.S., Uatu the Watcher, Nick Fury, Maria Hill, Heimdall, Guardians of the Galaxy (Star-Lord, Rocket Raccoon, Gamora, Drax the Destroyer, Groot).